Journal: bioRxiv

Article Title: Neuregulin-1 protects against respiratory viral induced mortality

doi: 10.1101/2023.05.10.540232

Figure Lengend Snippet: ( A ) CD11c + cells are increased in the lungs of atopic mice. Flow cytometry of lung cell suspension showing frequency (left) and cell number (right) at day 3 post inoculation (PI) high dose SeV ( B ) Adoptive transfer of CD11c + cells isolated from NA or atopic mice into naïve mice 24 h before inoculation with high dose SeV delays but does not prevent mortality; n=4 per group. ( C ) Transcriptomic (RNAseq) comparison between FACS isolated lung CD11c + cells from atopic and NA mice identifies several disparately expressed gene products, including Nrg1 (n=4 per group). Selected gene products shown. ( D ) NRG1 is markedly increased in atopic mouse lung (“tissue”), BAL, and supernatant from 1 x10 6 atopic lung CD11c + cells (“CD11c sup”) cultured for 24 h. ( E ) The scRNA (10x Platform) tSNE coordinates show CD14 + monocytes and dendritic cell (“DC”) sub-populations expressing NRG1 in peripheral blood cells of healthy human donors.*p<0.05, **p<0.01, ****p<0.0001

Article Snippet: Cell culture basal media was supplemented with recombinant human NRG1 alpha (cat#: NBP2-35093, Novus Bio) for hBEC or with mouse NRG1 for mTEC on day five and three before and at the time of infection with 4000 pfu of rgRSV or SeV-GFP.

Techniques: Flow Cytometry, Suspension, Adoptive Transfer Assay, Isolation, Comparison, Cell Culture, Expressing

Journal: bioRxiv

Article Title: Neuregulin-1 protects against respiratory viral induced mortality

doi: 10.1101/2023.05.10.540232

Figure Lengend Snippet: ( A ) NRG1(1ng to 1000ng) i.n. (in 30µL) given daily to naïve mice for 5d before inoculation with high dose SeV reduces viral mortality; n=4 per group (1ng, 10ng 1000ng & PBS), n=8 per group (100 ng and 500 ng). ( B ) Ratio of EBD in the BAL to that in the lung shows reduced EBD in NRG1 treated mice on day 8 PI SeV. n≥8 per group, median ± IQR shown, Mann-Whitney U test.

Article Snippet: Cell culture basal media was supplemented with recombinant human NRG1 alpha (cat#: NBP2-35093, Novus Bio) for hBEC or with mouse NRG1 for mTEC on day five and three before and at the time of infection with 4000 pfu of rgRSV or SeV-GFP.

Techniques: MANN-WHITNEY

Journal: bioRxiv

Article Title: Neuregulin-1 protects against respiratory viral induced mortality

doi: 10.1101/2023.05.10.540232

Figure Lengend Snippet: ( A ) Adding NRG1 to hBEC inoculated with rgRSV (left) and mTEC inoculated with GFP-SeV (right) reduces spread of infection. Representative images shown. ( B ) Quantification of (A) for rgRSV and hBEC and ( C ) for GFP-SeV and mTEC. GFP positive cells quantified by ImageJ. Representative images from ≥3 separate experiments. *p<0.05, **p<0.01. ( D ) Transcriptomic analysis of hBEC cultures treated with NRG1 (100 ng) on the basolateral side of the Transwell for 5 days and inoculated with RSV (4000 pfu). RNA was isolated 48 h PI RSV and qRT-PCR performed using a custom Prime PCR array plate: (i) Transcripts in which NRG1 treatment reduced gene expression from that seen in RSV infected cells. (ii) Transcripts with low level expression that show small but significant change in expression relative to naïve control with NRG1 alone or genes significantly increased with RSV but whose expression levels were not affected by NRG1. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, n=3.

Article Snippet: Cell culture basal media was supplemented with recombinant human NRG1 alpha (cat#: NBP2-35093, Novus Bio) for hBEC or with mouse NRG1 for mTEC on day five and three before and at the time of infection with 4000 pfu of rgRSV or SeV-GFP.

Techniques: Infection, Isolation, Quantitative RT-PCR, Gene Expression, Expressing, Control

Journal: Nature Communications

Article Title: Mapping of mitogen and metabolic sensitivity in organoids defines requirements for human hepatocyte growth

doi: 10.1038/s41467-024-48550-4

Figure Lengend Snippet: a Heatmap displaying gene expression patterns of cytokines, chemokines, and growth factors upon FH organoid growth from tissue. The mean expression of n = 2 donors is visualized as row Z -scores. b Comparison between the temporal mRNA expression profiles of selected cytokines and growth factors upon hepatocyte organoid growth from tissue (black) or single FHs (green). The mean expression of n = 2 donors per dataset is plotted (normalized transcripts). c Experimental strategy to evaluate the effect of cytokines and growth factors on human FH organoid growth. d Representative brightfield images of organoid-derived single FHs at day 0 and the outgrowing organoids challenged with indicated cytokines and growth factors at day 7. Representative of n = 2 challenge experiments. Scale bar = 100 μm. e Quantification of the organoid diameter 7 days post organoid outgrowth from single FHs under the different challenges ( n = 40 organoids per condition). Mean ± SD is plotted. EREG: * p = 0.0143; AREG: *** p = 0.0005; NRG1, IL1β, IL6, IL10, IL11: *** p < 0.0001, all versus control, one-way ANOVA with Dunnett’s post hoc test. Source data are provided as a Source data file.

Article Snippet: To optimize PHH organoid growth, different conditions were tested, including supplementation of the FH expansion medium with combinations of 100 ng/ml IL6, 20 ng/ml IL11, 20 ng/ml IL1β, or 100 ng/ml NRG1, and 10 μM cilofexor (GS-9674, FXRa) (MedChemExpress, HY-109083).

Techniques: Gene Expression, Expressing, Comparison, Derivative Assay, Control

Journal: Neural Regeneration Research

Article Title: miR-21 promotes the differentiation of hair follicle-derived neural crest stem cells into Schwann cells

doi: 10.4103/1673-5374.131599

Figure Lengend Snippet: miR-21 expression during the differentiation of neural crest stem cells (NCSCs) into Schwann cells. (A) Flow cytometry for differentiated NCSCs. (B) Quantitative real time (RT)-PCR tests showed that miR-21 expression was gradually increased with prolonged time ( i.e ., 10, 20, 30 and 40 days of induction by neuregulin-1). All measurement data are expressed as mean ± SD. The experiment was repeated in triplicate. One-way analysis of variance and Student-Newman-Keuls test were used for comparison between groups. * P < 0.05, vs . 0 day.

Article Snippet: Briefly, neural crest stem cells were collected and treated with MesenPRO medium (Invitrogen) containing 20 ng/mL neuregulin-1 (Sino Biological) in a 5% CO 2 incubator at 37°C for 28 days.

Techniques: Expressing, Flow Cytometry, Quantitative RT-PCR

Journal: Neural Regeneration Research

Article Title: miR-21 promotes the differentiation of hair follicle-derived neural crest stem cells into Schwann cells

doi: 10.4103/1673-5374.131599

Figure Lengend Snippet: Regulatory role of miR-21 in the differentiation of neural crest stem cells. (A) Flow cytometry showed S100- and GFAP-positive cells in the agomir-21, agomir-NC, antagomir-21 and antagomir-NC groups after 30-day induction by neuregulin-1. (B) Quantitative RT-PCR detected S100 and GFAP mRNA expression in the agomir-21, agomir-NC, antagomir-21 and antagomir-NC groups after 30-day induction by neuregulin-1. It took S100 and GFAP mRNA expression in the control group as 1. All measurement data are expressed as mean ± SD. The experiment was performed in triplicate. One-way analysis of variance and Student-Newman-Keuls test were used for comparison between groups. * P < 0.05, vs . agomir-NC group; # P < 0.05, vs . antagomir-NC group. agomir-21: miR-21 agonist; antagomir-21: miR-21 inhibitor; GFAP: glial fibrillary acidic protein.

Article Snippet: Briefly, neural crest stem cells were collected and treated with MesenPRO medium (Invitrogen) containing 20 ng/mL neuregulin-1 (Sino Biological) in a 5% CO 2 incubator at 37°C for 28 days.

Techniques: Flow Cytometry, Quantitative RT-PCR, Expressing